I am looking for a negative control mAb that does not bind to bacterial ATP synthase subunit beta protein in Western blot under denature and/or native conditions.
I have tried 7b2, synagis, CH65, and IVIG.
Highly appreciate your help.
I'm not sure exactly what you are asking, can you elaborate more on the type of control you would like to use?
Hey Khalid, sorry for the confusion.
I am looking for a monoclonal antibody that does not bind to bacterial ATP synthase beta subunit recombinant protein (species Aggregatibacter actinomycetemcomitans).
I plan to use this antibody as a negative control for my western blot in which I test the binding of another monoclonal antibody to this same synthase protein. Just to make sure that if there is any binding of my experimental mAb, it is not non-specific.
Thank you.
Use control isotype antibody, ie Mouse control IgG. It is generally taken as negative control.
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