Khalid Eldahan

4 Questions 27 Answers 0 Followers

Questions related from Khalid Eldahan

Quantifying the number of synapses in fixed tissue-how much does tissue thickness matter?

I am pursuing a project that will require me to quantify excitatory synapses in rat spinal cord slices (fixed in PFA). I have a number of references and protocols which describe methods for...

04 April 2017 8,460 1 View

How quickly are lentiviral vectors transported in the central nervous system?

I'm trying to estimate the amount of transport time needed to map out specific circuits in the spinal cord using a rabies-glycoprotein pseudotyped lentiviral vector. Does anyone know the kinetics...

01 January 2017 8,977 1 View

What are the important considerations in detecting large M.W. proteins using SDS-PAGE western blot?

I am intersted in detecting the p-mTOR protein, which is around 290 kDa. Can anyone give me some tips on how to efficiently blot for proteins in this size-range? At the moment, I am using 4-20%...

07 July 2015 1,753 7 View

Has anyone successfully stained rat spinal cord cross-sections with an antibody against p-NR1?

I have encountered issues performing fluorescent IHC on rat spinal cord cross-section to detect p-NR1 (SER 896). The antibody used in "ABN88" from millipore. While I do see positive signal...

06 June 2015 7,212 2 View