Quang Nhat Nguyen

5 Questions 6 Answers 0 Followers

Questions related from Quang Nhat Nguyen

How to quantify pixel density of a immunoblot?

Hi, I want to determine the pixel density of an immunoblot. Using ImageJ, I obtained Mean Gray Value and Integrated Density, but neither value really matches with what we observed on the...

06 June 2018 7,171 1 View

Does NFkB get phosphorylated in the nucleus?

Hello, I understand the general mechanism of how NFkB gets liberated from its IKK to enter the nucleus. But, does NFkB itself in T cells get phosphorylated when it was still in cytoplasm, once...

05 May 2018 1,345 4 View

What are key factors to ensure the most optimal protein expression/production/purification from both project management and technical perspectives?

Hi, From your experiences, what are some key technical considerations and project management best practices in ensuring the most optimal protein expression/production/purification? Thank you...

04 April 2018 8,058 5 View

How to best represent amino acid alignment data?

Hi everyone - I have a large dataset of aligned single-genome analysis (SGA) amino acid sequences from multiple species, tissues, and timepoints. I would like to visualize the differences among...

01 January 2017 458 4 View

Does anyone have any suggestion for a monoclonal antibody that does not bind at all to bacterial ATP Synthase subunit beta?

I am looking for a negative control mAb that does not bind to bacterial ATP synthase subunit beta protein in Western blot under denature and/or native conditions. I have tried 7b2, synagis, CH65,...

08 August 2015 5,106 3 View