Do extracted cell lines from 100% or more percentage of cell confluence (MC4-L2) have the optimal tumor generation effect?

I do MC4-L2 cell culture which is at ~100% or more of cell confluence. If I extract them and want to inject to mice, could they have their efficiency in tumor generation? Are they suitable for...

05 June 2015 7,582 5 View

How many MC4-L2 cell lines do you suggest for injection into one Balb/c mouse?

05 June 2015 8,018 10 View

Does anyone have any reference about the role of cytokines on fatigue/exercise-induced fatigue ?

Does anyone have any references or literature about fatigue mechanisms which cytokines are involved in it in none-disease subjects? Does anyone have any references about impact of cytokine on...

11 December 2014 9,264 6 View

Which of the breast cancer cells are less invasive and which ones are the most invasive? Can anyone help me ?

Which of the breast cancer cells are less invasive and which ones are the most invasive, do you think? I would like to know more about the mentioned question. Can anyone give advice, document or...

07 August 2014 7,346 17 View

What method is better to assay T lymphocyte telomere length?

I want to use the method (T lymphocyte telomere length assay) which has a higher accuracy and validity compared to other methods and in addition to these features, It is allocated the minimum time...

05 June 2014 9,749 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View