There are many kits on the market to measure PDE activity. I'm curious if anyone recommends one over another or has had good luck with a specific one.
Thanks,
did you try with bis(pNP) (or pNPP)? I never tried with PDE5A but it works with other phosphodiesterases
Hi Carlo, I'm not familiar with the methods you mentioned. Do you have any further information you could provide? A protocol or publication? Thanks
pNPP - https://www.neb.com/products/p0757-p-nitrophenyl-phosphate-pnpp#tabselect0
bis-pNPP - http://www.sigmaaldrich.com/catalog/product/sigma/n3002?lang=it®ion=IT
You can find tons of informations at these links, and even more googling the two molecules. Also, you can always check here for informations about enzymes http://www.brenda-enzymes.org/
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