Does anyone have any idea how long we can keep the bacterial isolates on agar medium (say in freezer) but remain highly usable for sequencing step?

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No title

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George Tetz

As you need DNA there is no real limitaion for that excluding the fact that it may become contaminated with time. Is o\not it easier to extract DNA and to keep it -20C?

Mohamad Sufian So'aib

Thank you. Yes, keeping the DNA extract is the best way. I just wonder in case we have problem with the DNA extract we could return to original sample to recover the DNA