13 Questions 23 Answers 0 Followers
Questions related from Mohamad Sufian So'aib
I conducted toxicity study on herbal product using zebrafish embryo. Once obtaining the LC50 of the product, I wish to get the HED value to imply safety of the product on human.
14 June 2019 7,468 2 View
In phylogenetic tree, I notice that a certain designation (consisting of letters & no.) are written after the name of the species. How does this designation created? Is it the accession no. of...
27 February 2019 4,810 5 View
I am trying to use 0.05% w/v L-csyteine as reducing agent to improve anaerobic condition of my agar media. I would like to know the proper way of doing this since logically protein would denature...
21 September 2017 8,251 3 View
I prepared Trolox working solution at different concentration - 0, 200, 500..800 uM in 10 ml abs MeOH. DPPH was used as antioxidant assay. For antioxidant activity procedure, 0.15 ml of each...
18 August 2017 9,400 13 View
I performed DGGE recently. After cutting out bands of interest, I eluted the DNA in 50 ul elution buffer containing 0.1TE and stored them at 4'C. How long the eluted DNA can be stored without...
18 May 2017 7,363 8 View
My PCR product of fungal gDNA did not meet the recommended amount for DGGE (i.e. 30-50 ng/5 ul way below 180-300 ng required). May I use DNA template of first PCR for subsequent PCR...
19 April 2017 4,486 14 View
I am currently working on fractionating mixed microbial DNA into individual species (which will be followed by DNA sequencing for identification). The mixed DNA was the product of several one-week...
15 April 2017 8,188 2 View
I found out different researchers used either MSTFA or BSTFA/TMCS as derivatisation of organic acid and sugar compounds. Since I only have BSTFA/TMCS at the moment, could I use it for both types...
24 March 2017 4,853 4 View
I read an article describing the use of super dehydrated EtOAc containing 2-ethyl-1-hexanol (0.01 mg/ml) in 400 ul mixture for volatile compound extraction. I tried to get appropriate product of...
12 March 2017 3,975 2 View
Our thermocycler breaks down recently. But due to constraints in getting complete specific reagents & consumables to conduct real time PCR, can we use real time PCR machine for normal PCR...
02 March 2017 3,848 3 View
My 80% MeOH aq extraction of a plant leaves followed by vacuum evaporation and N2 purge resulted thick wax. As most literature express gallic acid equiv (GAE) of total phenolic content (TPC) based...
28 February 2017 2,437 8 View
I am extracting papaya leaf & mangosteen rind using 80% MeOH. After vacuum evaporation, I could not get dry residue but thick waxy concentrate instead. I need to get residue to estimate total...
23 February 2017 9,645 3 View
Does anyone have any idea how long we can keep the bacterial isolates on agar medium (say in freezer) but remain highly usable for sequencing step?
17 December 2015 4,000 2 View
