I have exposed zebrtafish embryos to 3 toxicants of which 2 of the toxicants were individually added while the third one was a mixture of the first two. When I measured the intensity of the plate, the first compound didn't fluoresce, while the other two did.
My question: DCFH-DA can it be oxidized by other reactive oxygen species than hydrogen peroxide such hydoxyl radical, peroxy nitrile, etc? in other word, is DCFH-DA sensitive for H2O2 only? I have many papers regarding this issue yet I cant come to a conclusion. Can I please have some assistant and perhaps opinions for that matter.
Thank you all kindly. I very much appreciate it.
Sincerely,
OI
I can quote a review on redox fluorescent probes chemistry: 10.1016/j.freeradbiomed.2011.09.030.
Citation: “the DCFH-DA probe cannot be reliably used to measure intracellular H2O2 and other reactive oxygen species”. I am totally agree with this opinion. Unfortunately we made this mistake and spoiled some data, please do not repeat it unless you comprehend all possible artifacts of this dye.
More evidence on the problems associated with DCF in the attached paper.
Regards,
Christian
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