Please share only simple protocol like based on optical density basis and chlorophyll content basis.
Can you specify which type of algae,chlorophyceae,Bacillariophyceae, rhodophyceae?
I used optical density (cyanobacteria). First I scanned with the spectrophotometer the absorption, and I used a wavelength in which there wasn't any absorption peak. Then I grown a culture, and diluted it so I had 250 ml of culture 100%, 75%, 50%, 40%, 30%, 20%... (v/culture medium). I read the absorption using the wavelength previously determined for any of those, and I centrifuged 50 ml of those cultures in weighted tubes (you have to do it with by triplicate at least), eliminated the medium and dried it, till constant weight . Then I related the absorption to the weight.
If you do this, probably the curve will saturate, so you can only use the lineal part to get an useful equation.
Now, that you can relate absorption to weight you can do a growth curve.
You have to be careful with exopolysaccharide, and other products that could affect the weight.
thanks Marta, for sharing useful information please provide me some refrences of the above said and if you have idea about growth in relation with chlorophyll than please suggest ,because i am planing for Ph.D.
Dear, Tarkeshwar i am talking about Chlorella and Chlamydomonas. if you have any idea please share with me .
You can try Daily cell counting method and also you can try measuring OD at 665 day by day,for growth curve.
Hello people,
please tell me, do we really need to change the media of culture during growth curve?
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