Does anyone have an experience in 'en face' staining of murine aorta with NBT?

06 March 2020 1 8K Report

Hello. I am trying to test if optical fibre causes any thernal damage to murine aorta. The plan is to do longitudinal cut and stain the vessel after fixation with PFA. Does anyone have a prtocol which I could use?

Muna hussain Al-Aameli

A special dye of dethidfiocycine can be used to distinguish fiber.

Badges
Science method

More Elena Tsisanova's questions See All

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

Flow cytometry compensation with beads - is the matrix the problem?

I am trying to compensate my flow cytometry data. I have human tumor tissue, dissociated and stained with live/dead fixable::APC, EpCAM::FITC, CD45::PE. I also stained and measured single stain...

07 July 2024 5,614 5 View

Is it possible for the soil pH to be around 8, but with negligible amounts of carbonates, bicarbonates and exchangeable sodium?

Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!

26 June 2024 9,093 4 View

How do you culture THP1 Leukemic Stem Cells?

Hi! I have to test a drug on the LSC fraction of THP1, but I'm not finding any specific media to select and culture them. Do you know anyone? Thank you in advance

15 May 2024 1,464 3 View

Methanesulfonic acid neutralisation?

Hello, I am performing esterification reactions between fatty acids and alcohols, in presence of methanesulfonic acid and H3PO2 as catalysts. At the end of the reaction, to reach the acid index I...

22 April 2024 2,063 5 View

Method DIN EN 14110 ?

I need to analyze the residual methanol from methyl esters. I am wondering if there is someone who has this method DIN EN 14110 and is willing to share it with me? Thank you in advance, Elena

22 April 2024 387 0 View

What is the last resort washing solution for a pepsin column that has a blockage?

Recently my pepsin column got some blockage, supposedly because of the precipitation of aggregated protein. Multiple washes with 0.1% FA or pepsin wash (1.5M GuHCl + AcN + FA 95.2/4/0.8), as well...

20 April 2024 3,135 5 View

Why heptane is integrated in GC chromatograms ?

I am facing an issue with how chromatograms are recorded. From time to time, on our chromatograms the peak assigned to heptane is integrated as well and this gives us wrong results. Sometimes the...

07 April 2024 1,726 4 View

How can I calculate p-value from post hoc test for Kruskal-Wallis multiple comparisons in SAS?

I need to perform non-parametric post hoc multiple comparisons. I found two interesting macros that implemented methodology about post hoc test for Kruskal Wallis test, but p-value was never...

07 April 2024 288 3 View

What is the recommended sample size for the validation of a standard questionnaire consisting of 40 items?

Dear colleagues, I am looking for advice on the validation of a standard questionnaire that I intend to translate. The original version contains 40 items. Could you please tell me what sample...

26 March 2024 1,660 11 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View