Does anyone have an exhaustive list of the HNF1b target genes?

More Malamelli Lilia's questions See All

What is the difference between alluvial and fluvial?

I have made an unpleasant discovery that I seemed to use these terms incorrectly. 'Alluvial' seems to mean "out of the river channel", flood-plain' etc. While all these cross-bedded...

15 July 2024 2,073 3 View

What analysis can be performed to detect traces of acetone in extracts?

Use a solution of acetone and water to perform an extraction. After performing it, evaporate the acetone and lyophilize the extracts, but I am looking for a methodology to confirm that acetone has...

10 June 2024 5,047 3 View

Do you have Related Studies on the effect of Video-Aided Instruction in developing writing proficiency of learners?

I need journals or articles about the effect of video-aided instruction in developing writing proficiency of learners.

28 March 2024 2,045 0 View

Issue with Inappropriate Test Masks on ISIC 2018 Images?

Hello everyone, I am currently facing an issue with the test masks on ISIC 2018 images. It seems that the masks do not align appropriately with the images. Has anyone else encountered this...

05 January 2024 4,264 0 View

What's wrong with my exosome isolation protocol?

Hi! I'm working with MSC from Umbilical Cord for exosome isolation. I used beta amyloid to induce/increase the exosome production and normal culture conditions to compare. I'm using UC protocol:...

07 June 2023 5,764 2 View

How can I calculate the extracellular concentration of sodium (Na+) using the Nernst equation?

A membrane at room temperature has an internal Na+ concentration of 38 mM. What is the external concentration if the reversal potential ENa is 55 mV?

24 May 2023 6,372 0 View

How calculate the BDE value by gaussien ?

Good morning, I want to know how to calculate the BDE value of antioxydante by the logical Gaussian view?

20 September 2021 1,256 9 View

Does anyone know how to use the Conflict Tactics Scale short form to use it a linear regression model ?

Hi everyone, Does anyone know how to use the Conflict Tactics Scale short form (CTS2S; Straus & Douglas, 2004)? I am working on my master thesis and I am looking at Gender Role Ideologies as a...

14 September 2021 5,323 3 View

Сan anybody tell me a publication on a supplementary loop of M1 in the Late Middle Pleistocene Microtus, Northern Europe?

I remember that one time I came across a publication, where a frequent occurrence of M1 with the supplementary loop was mentioned. It might have been the Holsteinian of Netherlands (not for...

19 May 2021 7,452 5 View

Wharton's jelly mesenchymal stem cell isolation?

I have read and done several protocols for WJ-MSC isolation but none of them work. I have isolated some cells, but never enough for them to survive I cut and dissect 1-3 mm3 of the umbilical cord,...

23 September 2020 4,752 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View