How have professors been able to transition complex ideas and concepts to a virtual environment?

As COVID-19 has forced academia to react instantly to the dynamics of teaching and learning, I am curious to find out how you have been able to transfer complex ideas that you would normally teach...

17 May 2020 8,254 16 View

Can I activate a mixed primary (mouse) glial culture, and then shake microglia off?

Our lab usually uses the shaking method to isolate primary mouse microglia from mixed glial cultures. Till now, I have been collecting microglia, plating them in isolation, and then activating...

17 November 2019 2,048 1 View

Has anyone had issues with extracting RNA from serum-grown bacterial cells?

In a side-by-side experiment, I got a normal amount of RNA from cells grown in chelexed BHI but very low yields from serum-grown cells. There was more cells in the serum culture, so low cell...

10 May 2018 2,167 3 View

What is the best molecular marker to separate healthy and pathological astrocytes?

I have quantified astrocytes with GFAP in a series of westerns. I would like to know if the lower level of GFAP I am seeing is because it is non-reactive due to a healthy environment or...

06 September 2017 5,783 1 View

My error correction term/speed of adjustment be -1.60529.?

I have performed VECM test with LLB and CPS as my independent variable and GDP as dependent variable. Can i write that In the absence of changes in LLB and CPS deviation of the model from long run...

18 August 2017 684 1 View

Do you have experience or protocols for cryofixation and sectioning of fetal/embryonic pigs?

14 December 2016 2,639 4 View

I have a mixed-design (2: condition X 2: pre-post) but due to a problem with data collection am unable to match pre-test with post-test data. Help?

I am in a bit of a bind with a project evaluating a classroom-based intervention. The design was supposed to be a mixed-design whereby I collected pre-post data from willing students linking...

05 December 2016 938 4 View

How to limit LDH Oxidation during the extraction process?

Hello, I am doing research on the glycolytic pathway and have been getting unreliable results for my lactate dehydrogenase assays, due to LDH being subject to oxidation. Does anybody have any...

06 July 2016 846 1 View

Hi, does anyone have an idea on how to purify a molecule with free thiol function without risking its oxidation?

I am interested in getting pure molecules with free thiol but the problem is that during the synthesis I always get a mixture of free thiols and disulphide bridge. After the purification on...

24 May 2016 1,699 1 View

Does anyone have any information regarding a tool to measure attitudes and beliefs of clinical nurses regarding a specific clinical intervention?

Does anyone have any information regarding a tool to measure attitudes and beliefs of clinical nurses regarding a specific clinical intervention?

14 February 2016 5,014 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View