Can anybody help me with an efficient way to de-protect poly(tert-butyl norbornene-2-carboxylate)?

I want to make poly(norbornene 2-carboxylic acid), for the sake of characterization, I decided to start with the protected monomer norbornene-tert-butyl-2-carboxylate for the ring-opening...

03 December 2020 3,852 5 View

Why Schiff bases change colour upon evaporation?

I got bright yellow coloured Schiff base after 6 hour reaction time. So i kept it for slow evaporation. Solvent was methanol. But after complete evaporation my product colour changes from yellow...

17 November 2020 3,824 2 View

Has anyone observed delamination of pyrolysed SU-8 on SiO2 coated Silica substrates when the pyrolysis is performed outside cleanroom?

We have a quartz tube furnace (diameter 80 mm) outside the cleanroom and when tried to pyrolyse the SU-8 on SiO2 coated Si substrates at 900 C in nitrogen atmosphere, the electrodes looks fine....

08 November 2020 4,357 2 View

Is there any chance for synthesis Schiff bases using 4-methyl-5-imidazole carboxaldehyde?

Is there any chance for getting product using the above aldehyde and different amines.

12 July 2020 8,330 2 View

Can anyone help me finding good impact factor journals to submit uninvited review paper on postharvest chilling injury in fruits.?

I have tried submitting my review paper in relevant journals, but most of them only accept the review paper invited by the editor of the journal

28 June 2020 4,371 5 View

What is an alternate way to recover a polymer product from DMSO or DMF other than evaporation?

Due to the high b.p of DMSO and DMF (189 and 153 degree Celsius), it is difficult to recover my polymer from the solution. I believe extraction in a triple system (such as DCM, DMSO and water)...

04 March 2020 3,028 12 View

What were the active sites on the magnetic activated carbon surface for adsorption of reactive dyes? Were they carbon atoms or Fe3O4?

In the adsorption process by using adsorbent magnetic-activated carbon for removal of reactive blue 13 dye which contains -N=N- group, SO3 group, OH, Cl, etc. In this process what are the active...

24 February 2020 1,110 4 View

Why i am getting only one peak at 280 nm for dyes ?

I prepared aminobenzothiazole reacted with amino phenol azo dyes but on taking UV I got single peak. Why I couldn't get 2 peaks?

18 February 2020 6,949 3 View

Could anyone please help me to get my schiff base product?

Hereby I am explaining the detailed procedure which i done in my lab. 1:1 molar ratio of 2-amino benzothiazole and 4-nitro benzaldehyde were refluxed for 12hr at 100 degree Celsius in toluene...

24 January 2020 5,094 3 View

Is it possible to determine the molecular weight of emulsion polymers by GPC?

I performed an emulsion polymerization of tert-butyl acrylate using KPS as initiator. I confirmed the formation of polymer by NMR. But when I tried to determine the molecular weight using GPC, GPC...

12 January 2020 6,711 8 View

How could the concentration from RNA extraction is really low?

Hello everyone, currently, I'm trying to do RNA extraction from seeds of Arabidopsis. I'm using the protocol below: RNA isolation from siliques, dry seeds, and other tissues of... I use about...

17 February 2021 7,583 4 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View

What is the method of removing RNase contamination from a filter paper surface?

I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...

12 February 2021 3,926 3 View

Buillding 3D Model Fussion Protein with Modeller?

Hello May i ask you about using Modeller for making 3D model fussion protein. I want to build a fusion/chimera protein structure which is including 2 different protein and then evaluate quality...

09 February 2021 9,369 2 View

Sequence of an unknown Oligonucleotide ?

Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .

04 February 2021 5,614 3 View

How to make RNA extraction process RNase free inexpensively?

We want to extract RNA from virus samples, so we need to make everything RNase free. However, DEPC or RNase cleaning products are out of the question for us at the moment because of their cost. Is...

02 February 2021 2,343 4 View

How can i improve my DNA purity for whole genome sequence that has high 260/230 ratio ?

I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...

31 January 2021 355 2 View

RNA extraction from exocrine pancreas?

Hi everyone ! Does anyone have any experience in extracting RNA from exocrine pancreas (which is very high in RNAse)? The pancreas is frozen tissue. I wonder if RNAse is also high in the nucleus,...

31 January 2021 390 2 View

Suggestions for using antisense oligonucleotide for brain delivery?

hello I am working on a project that includes gene delivery to the brain. my intentions are encapsulating a gene in a Chitosan NP and IV administration the NP to rats. beside plasmids is there...

29 January 2021 4,455 5 View

Can we incorporate unnatural amino acids into protein using software for graphical image purpose?

I am looking to incorporate a unnatural amino acids which is not available in the swisssidechain database into a protein crystal structure of pdb? I am using chimera 1.14 vis. tool?

26 January 2021 4,064 3 View