What concentration range should I use to build standard curve for ortho-nitro phenol ?

I would also like to know the linear range of this assay and the detection range? This will be used to measure end point production from beta-gal assay.

26 March 2018 1,546 3 View

Can anyone suggest unique protein marker for both single and double stand breaks for cell line study?

I would be studying the cell line and in one experiment the correlation of the damage to the cell ( DNA treatment) should be correlated to the concentration of the dose. I do not have western...

15 January 2018 3,210 3 View

How to distinguish between corroded surface morphologies prior to crack initiation for (i) pure corrosion and (ii) corrosion fatigue conditions ?

In a continuously or intermittently moving component like a compressor blade in an aeroengine, corrosion may occur both at static as well as in the running state. By this, I mean that corrosion...

30 November 2017 5,359 3 View

Hi everyone. For RNA viruses, what are the procedures for whole genome sequencing directly from cDNA without amplification using specific primers?

09 August 2017 6,191 3 View

Is there any free software for windows 10 where I can design a microstrip patch antenna array?

is there any link of hfss or zeland ie3d for windows 10

20 March 2017 9,343 3 View

How to model a protein with multiple domains and no template covering more than one domain?

I'm trying to model a protein which has multiple domains and templates available covering small parts of the sequence. It is a 4500 aa protein and to moderately covering the sequence it needs 7...

11 December 2013 9,126 19 View

Can someone recommend a good theoretical method by which two mutant variants could be differentiated?

Stability of protein mutants: I'm trying to design thermostable variants of a protein. Is it possible to theoretically differentiate them without doing simulation? What would be the parameters I...

21 July 2013 5,401 4 View

How to relate loop flexibility with enzyme catalysis?

I'm working with an enzyme where some of the loops are playing important roles in catalysis. How could I study the relation of loop flexibility with the re-activity (computationally)?

09 June 2013 9,940 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View