I collect fish muscle and small fin pieces for population and landscape genomics studies. I have been using cold preservation with liquid nitrogen or dry ice canisters. But sometimes the areas are very remote, which affects the cold chain. I could use a buffer that allows transport at room temperature. I have been reviewing that some use RNAlater, NAP buffer, DESS, or 95% Ethanol.

  • Which of those or others do you recommend in terms of DNA degradation?
  • Are there any problems extracting DNA later?
  • How to avoid PCR inhibitors with that buffer you recommend?
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