I am trying to express toxic peptides using a bacterial expression system. Can someone share me an experience/ or any possible strategies for successful production of toxic peptides in bacteria?
Dear Alemu,
It really depends on what kind of issues you are facing while you are doing your toxic peptides expression. I can immediately think of two common strategies:
1. Culture your bacteria in lower temperature.
2. Using some specialized bacteria strains that are commercially designed for toxic protein expression. Eg. C41 and C43 from Lucigen.
It depends on toxicity of your peptide and how it's function, then you must used mutant strain that your peptide haven't any toxicity effect on that or you can use fungal or mammal cell for cloning and expression.
In lac operon controlled bacterial T7 expression system, Glucose is used to suppress the basal expression of the toxic protein and additionally pLysS containing host is used. When expression is required, glucose deficient media is used and IPTG is added.
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