Does the pre-induction OD600 affect expression of stable proteins from bacteria?

We often induce protein expression when OD600 of E. coli culture reaches 0.4-0.8. What is/are the effect(s) of the pre-induction OD600 on expression level and stability of target proteins from...

02 March 2018 639 2 View

What is/are the best method (s) for separation of co-expressed chaperones from recombinant proteins?

Co-expression of chaperones with recombinant proteins often enhances expression of soluble proteins. However, separation of some co-expressed chaperones from target proteins sometimes becomes a...

08 September 2017 1,014 3 View

I am able to detect DNA bands on agarose gel from negative controls of PCR products? Did any one comeacross the same problem?

Recently, I have performed PCR reactions to amplify different gene fragments for cloning. I usually included negative controls in my experiments. The negative controls contain all the reaction...

07 August 2017 2,415 10 View

Does anyone have a strategy for production of recombinant toxic peptides using a bacterial expression system?

I am trying to express toxic peptides using a bacterial expression system. Can someone share me an experience/ or any possible strategies for successful production of toxic peptides in bacteria?

07 August 2017 336 4 View

I detected three distinict bands of a purified plasmid on agarose gel. Do you think the plasmid has been degraded?

I have purified a plasmid DNA using a plasmid miniprep kit for transforming into a bacterial expression system. I run an agarose gel for detection of the purified vector. I could detect three...

07 August 2017 3,211 4 View

Is it possible to engineer E. coli strains that do not produce endotoxins?

E. coli strains are good working horses for expressions of recombinant proteins. They have got huge potential for production of low-cost subunit vaccine candidates against various target diseases....

05 June 2017 2,241 12 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View