Does anyone have a standardized mitotracker assay for zebrafish embryos?

More Benan John Mathai's questions See All

Whether anyone know about Healthcare journal (MDPI publishers) impact factor??

Is MDPI publishers a reputable one?

15 September 2017 2,436 9 View

Which are the journals(e health) without ant publication fee?

I am looking for a journal which does not impart any publication fee. Can someone help if they know

03 April 2017 622 3 View

How can I be sure about my co-IP results that were done using Dynabeads protein G?

I have been trying to do co-IP to determine the interaction between my interested protein with unknown protein. I have two negative control that are beads only and non-spesific IgG. Since I used...

22 February 2016 3,134 3 View

Is it advisable to store isolated RNA in RNAlater?

Well, I have always used RNAlater for preserving tissue samples or embryos, for isolation of RNA at a later time period or even for protein extraction at a later time period. But I recently...

12 October 2014 4,889 5 View

What significant bioinformatics work can be done with a gene sequence of unknown function?

I have done some basic analysis.

01 January 2014 5,313 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Domenico Cieri

Hi, I suggest you to give up with this strategy. The only way to label mitochondria is, in my opinion, through the injection of plasmids encoding for fluorescent probes targeted to mitochondria. I've been working for three years with zebrafish mitochondria, and this is the best way I found. Mitotracker is inefficient to penetrate tissues deeper then skin.

Benan John Mathai

Thanks Domenico!! Even I have given up on that stuff!! :)

Somaieh Ahmadian

i am glad that i read this just after two weeks of trying!

Roberto Espinosa-Vallejo

Domenico Cieri could you give some references about the difficulties you undergo in mitotracker assay for zebrafish embryos. Have you seen does problems in early embryo stages to?