Dissolving higher concentrations of L-DOPA?

More Benan John Mathai's questions See All

I need a protocol for PI staining using Ethanol as a fixative. Online protocols are to do with kits and I have only PI powder. Can anyone share?

I need to do fluorescent microscopy using Propidium Iodide. I initially fixed my cells with 4% paraformaldehyde and saw red stain in Control cells. It turns out PFA is cell permeable. So if anyone...

27 February 2024 437 3 View

Can the nebulization gas pressure in ELSD affects the retention time of analytes?

I am developing a method for FAMEs using normal phase chromatography and ELSD as detector. I observe shift in Retention times (more retention) for subsequent injections. also there is a minor...

30 August 2020 7,414 7 View

How I can get procedures for Urinary Fatty Acid Methyl Esters using GCMS?

Urinary Fatty Acid Methyl Esters using GCMS

15 April 2019 6,583 0 View

Does anyone works in single pixel imaging ?

I do experiments in single pixel imaging to for the recontruction of transparent object . I use DLP6500 digital micromirror device and PDA36A2 detector for the acquisition process ,but i am not...

15 October 2018 7,206 0 View

Whether anyone know about Healthcare journal (MDPI publishers) impact factor??

Is MDPI publishers a reputable one?

15 September 2017 2,558 9 View

Which are the journals(e health) without ant publication fee?

I am looking for a journal which does not impart any publication fee. Can someone help if they know

03 April 2017 727 3 View

Where can I find low velocity drop weight impact testing in india for aluminium hollow box to find the energy absorption under impact loading?

The hollow box size is 50*50 and the height is 37mm.I want the load vs displacement graph.

26 February 2017 9,950 1 View

How can I be sure about my co-IP results that were done using Dynabeads protein G?

I have been trying to do co-IP to determine the interaction between my interested protein with unknown protein. I have two negative control that are beads only and non-spesific IgG. Since I used...

22 February 2016 3,150 3 View

How will thermal or electrical properties of work piece material will affect the wear on the tool electrode in Electro Discharge Machining Process?

In EDM process, tool wear is almost inevitable and wear characteristics do depend on tool electrode material properties, flushing conditions etc. But will tool wear be affected by the work piece...

10 February 2015 4,345 1 View

Is it advisable to store isolated RNA in RNAlater?

Well, I have always used RNAlater for preserving tissue samples or embryos, for isolation of RNA at a later time period or even for protein extraction at a later time period. But I recently...

12 October 2014 4,908 5 View

How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.

26 July 2024 1,469 1 View

How do you prepare a solid sample of biosynthesized Silver Nanoparticle (AgNP) for a UV-Vis spectrophotometer?

I have synthesized AgNP prepared from a plant leaf extract and AgNO3 solution. It underwent optimization by applying different parameters (reaction time, pH, concentration, etc). The solid NPs...

12 April 2024 6,042 2 View

How can I optimize the pH of biosynthesis AgNP? Is it acceptable to use chemical reagents to adjust the pH in green synthesis approaches?

I am planning to synthesize AgNP using plant extract as a bioreductor. I have come across several papers recommending a neutral to alkaline pH range (pH 7-8) to achieve silver nanoparticles with...

19 March 2024 1,246 4 View

What is the correct way to prepare a 1 mM AgNO3 solution ?

I have come across suggestions for optimizing the biosynthesis of silver nanoparticles to achieve smaller particles (

19 March 2024 7,054 5 View

Why does Tyrosinase suddenly become inactive when it was active previously?

Hi all. I ran the enzyme activity (pure Tyrosinase after purification using recombinant e coli) using L-Dopa with this condition 50 mM phosphate buffer ph 6, 1mM L-Dopa 25 C temperature 0.5 mM...

06 February 2024 8,350 4 View

Can it be non-enzymatic digestion of BAEE in enzyme kinetics determination experiment?

Have anybody measured a kinetics of enzymatic digestion of BAEE(n-alpha-Benzoyl-Arginine Ethyl Ether) with USP method? I have observed acceptable delta-OD when use significant amounts of enzyme...

24 December 2023 9,368 1 View

Are metabolically engineered strains genetically modified organisms?

Metabolically engineered strains in the sense of overexpression or deletion of specific genes within their metabolic pathways, enabling the transfer of biosynthesis pathways between different...

19 December 2023 6,822 1 View

Someone have a precise protocol to perform DAF-FM Fluorescence in HUVEC cells ?

We are currently having some problems with DAF2 or DAF-FM fluorescence. We use a 5µM concentration and are not able to see any effect of a stimulation on DAF fluoresnce. When looking cells under...

16 November 2023 8,182 0 View

Can Rhizobitoxine inhibit growth of cereals like Germination-Arrest Factor -1 (4-formylaminooxyvinylglycine)?

Rhizobitoxine, produced by Bradyrhizobium sp. , Rhizobium japonicum, and Robbsia andropogonis, was recognized as an inhibitor of methionine biosynthesis. This compound is phytotoxic to soybean and...

16 November 2023 5,074 0 View

Does addition of L-arginine into DMEM increases the production of nitric oxide by RAW264.7 cells after LPS treatment?

If yes, what is the ideal concentration of L-Arginine in DMEM?

04 October 2023 1,856 0 View