Domenico Cieri

3 Questions 8 Answers 0 Followers

Questions related from Domenico Cieri

Is anyone familiar with Gateway LR reaction giving tons of colonies?

I performed two LR reactions to generate an expression vector to express in zebrafish (using the Tol2 system). I am very familiar with this since I generated a lot plasmids with this approach in...

07 October 2016 4,033 3 View

Can GFP-fusion to N-terminal alter native protein function?

My question is pretty general so I don't know if anybody could help me. Briefly I have overexpressed (in zebrafish trhough a UAS plasmid) a fusion protein created by fusing GFP to the N-terminal...

10 October 2014 3,173 4 View

How to demonstrate protein overexpression in zebrafish?

I'm having big issues demonstrating overexpressoin of my protein in zebraifsh. I have been injecting mrna for two years, making experiments with this mrna and finding interesting data. Since I did...

22 August 2014 8,547 10 View