The longest fragment I can find is from ThermoFisher (NoLimits 20,000 bp, p/n SM1541). Does anyone know of larger, purified DNA oligonucleotides that are commercially available?
lambda DNA is ~50kb as far as I recall. If you hit that with a rare cutter (NheI, XbaI cut once), you'll get what you might be looking for.
Geoff,
Thanks for the suggestion. Our labs are not very biological in design and tend towards RF generators, acoustic transducers, and mechanical engineering. That is why I was trying to find a commercial source. I may just have to go into the lab and do some biochemistry but I was hoping to stay away from that path. Harder yet are the 100 kbp and 150 kbp fragments. I suppose that I could do even more biochemistry and concatenate lambda fragments but that is really outside my expertise.
I hope that someone comes up with a commercial source.
In our lab we use a CT DNA (from Sigma) as source of big fragments (more than 20 kb). I presume it is a mix of the fragment of different length. On gel the smear band is higher than the biggest ladder band (12kb). Potentially you can also try and purify long DNA or try to concatenate.
Thanks, Nazarii,
As I mentioned to Geoff, we are looking for very large fragments of 100,000 bp to 150,000 bp.The "NoLimits" fragments from Thermo were great but stopped at 20,000 bp. As a physics lab, we do not have the facilities or expertise to concatenate and purify our own fragments. That is why we are searching for a commercial source. The CT DNA fragments from Sigma are just too small for our needs.
Anybody else have an idea?
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Pharmaceuticals
- Drugs