Hello friends,
I am attempting to separate E-Cad+ normal mouse epithelial cells from E-Cad- mouse tumor cells using the difference in E-Cad expression. I have tried several antibodies but have yet to find one that can bind specifically to live epithelial cells. Ideally, I am looking for antibody I can use for cell sorting but at this point I will take even recommendations for an anti-E-Cad antibody with good specificity for IF applications. I appreciate all and any suggestions!