There is a very strong association between the two since most ROS are synthesized in the mitochondria and the level of function/dysfunction of the mitochondria also plays a significant role in the level of antioxidant production.
Are you investigating a specific mitochondrial disease? Determination of the antioxidant enzymes such as glutathione, catalase and superoxide dismutase can be done easily from the blood using commercial kits or simple laboratory protocols.
These references will explain further how the whole scenario works
https://www.uam.es/personal_pdi/ciencias/genhum/biblioparte2/SEMIN6.pdf
http://www.hindawi.com/journals/jst/2012/646354/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102435/?report=classic
http://scholar.google.com.my/scholar?q=mitochondrial+disease%2Boxidative+damage%2Bdisease&hl=en&as_sdt=0&as_vis=1&oi=scholart&sa=X&ei=OUE2VNX3EJSWuQSjmYKABw&ved=0CBkQgQMwAA
Regards,
You can check the level/activity of antioxidant enzymes in the mitochondrial fractions.
There is a protocol for mitochondrial isolation and the estimation of antioxidant enzyme levels. Please refer the following link for further details.
https://www.researchgate.net/publication/216540220_Ellagic_acid_protects_mitochondria_from_-adrenergic_agonist_induced_myocardial_damage_in_rats_evidence_from_in_vivo_in_vitro_and_ultra_structural_study
http://ac.els-cdn.com/S0963996911005515/1-s2.0-S0963996911005515-main.pdf?_tid=8f70b0cc-4f8e-11e4-b1fc-00000aacb35e&acdnat=1412843624_2980339a69108a292dd481cf4eb5373a
Article Ellagic acid protects mitochondria from β-adrenergic agonist...
I wonder how do you distinguish between ROS produced by mitochondria from ROS produced by others (say, by polymorphonuclear neutrophils at the inflammation site)?
We have two kits that may be helpful. To measure the overall antioxidant activity of the serum, you could use the serum to quench superoxide and hydrogen peroxide. In this case, our Diogenes Kit may be usefull along with the Xanthine/Xanthine oxidase protocol in the instructions (https://www.nationaldiagnostics.com/oxygen-assay/product/diogenes), as a means of measuring superoxide quenching; and you could use our hydrogen peroxide assay kit to measure quenching of a known amount of peroxide.(https://www.nationaldiagnostics.com/oxygen-assay/product/hydrogen-peroxide-assay-kit)
Yes there is a correlation. Use ETC inhibitors and check the modulation in ROS using mitosox red. Also simultaneously monitor the changes in antioxidant levels. You will definitely get your answer.
Regards
- Badges
- Science topic