I can purify the lysosomes just fine. I just need a recommendation of parameters for sonication. Thanks in advance!
Dear Lance, for lysosome lysing without surfactants, you can try mechanical methods based on using a high-pressure homogenizer, liposome extruder or repeated freezing/thawing.
I found this advertisement from a farm in Indonesian selling what sounds like desiccated Sechium edule seeds (Chayote). I have always read that it is impossible to dry chayote seeds and then...
30 August 2020 1,274 3 View
I know there must be a formula. I'd prefer not to use NaOH if possible.
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I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...
03 March 2021 1,931 5 View
In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...
01 March 2021 1,960 4 View
Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...
01 March 2021 9,332 6 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...
25 February 2021 2,225 5 View
I'm trying to find in literature a way to help me adding a modified Yen's algorithm in ns2 or its variance ns3 or another simulator. Have you some tips which can be helpful, I'm opening in the...
19 February 2021 7,981 3 View
I want to apply a privacy-preserving algorithm in MQTT for IoT devices. Which tool do you suggest to simulate this algorithm? I want to measure the performance on the IoT battery device? Actually,...
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26 January 2021 3,847 7 View
Hi everyone, I'm doing metabolomics of placental tissue in my lab. I added 1ml of 80% MeOH along with 2 isotopic internal standards namely, phenylalanine and palmitic acid to my sample after...
18 January 2021 9,520 4 View
I am working on handoff management in IEEE 802.11p/WAVE based VANETs. I want to know if I can simulate V2I communication mode using NS-3, which will lead to the simulation of handoff process. Most...
16 January 2021 1,733 4 View