Does anyone have a protocol for lysing purified lysosomes using sonication? I want to avoid detergent because I will be using the lysosomal contents.

08 August 2017 1 7K Report

I can purify the lysosomes just fine. I just need a recommendation of parameters for sonication. Thanks in advance!

Dear Lance, for lysosome lysing without surfactants, you can try mechanical methods based on using a high-pressure homogenizer, liposome extruder or repeated freezing/thawing.

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