Why can I not enter the title of the journal?

When I upload articles the details page does not allow me to enter the names of lesser known journals. It says "type to search" but because the journal is not on the list, I am left with a blank...

26 January 2021 2,988 3 View

How do I calculate transition probabilities for a sequence of syllables in a set of bird songs using R?

I would like to automatically generate a Markov chain analysis for my set of bird songs that I have assigned syllable categories to using R. I have around 70 songs (each from an individual bird)...

13 October 2020 4,833 2 View

Does anyone have any recommendations for good, clean antibodies they have used for IHC in the mouse lung?

I am new to the immunohistochemistry world and am currently about to teach myself the basics on some sections of C57BL/6 mouse lung. Does anyone have any good antibody recommendations or proteins...

28 October 2019 602 3 View

Is there a published protocol for extracting lipids from skeletal muscle?

Hello, I am wanting to express mouse skeletal muscle contractile performance to lean tissue mass, and I had been advised in a piece of peer review for a paper that it is possible to perform such...

17 March 2019 716 1 View

Comparing Significant Slopes in Simple Slopes Analysis?

Hi everyone, I am running a moderated linear regression and I found a significant interaction effect. I then used the Preacher online calculator to conduct a simple slopes analysis. My simple...

09 March 2019 3,196 7 View

Radiative heat from surface?

Im calculating heat loss from tank surface that is located inside. Assuming ambient temperature is 293K and surface temperature is around 310K. Is it ok to assume that main heat transfer form is...

08 May 2018 2,909 4 View

My gel is showing two smeared lines very far apart, what could be the cause for this?

I'm looking at primers at different annealing temperatures and my gel is showing two distinct streaking bands at the top of the ladder and at the bottom of the ladder. I'm using DNA from ferns...

11 April 2018 5,809 4 View

Who has published on the dis-/advantage of being young for starting a business? Focus on calendar age and being young.

I am interested in publications on the impact of being young when starting a business and gaining clients; such as not being taken serious or the opposite of being seen as better than older...

11 June 2017 5,825 4 View

What is the differences between bottom-up and top-down proteomics approach?

17 January 2017 3,528 3 View

Does anyone have experience cloning GFP into lentiCRISPRv2 vector?

I want to insert GFP into my lentiCRISPRv2 backbone so that I can immediately see transfection efficiency and lentiviral infected cells instead of waiting for purokill. Does anyone have experience...

29 October 2016 7,333 3 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

Which database is the most suitable for checking English bigrams frequency?

Which database is the most suitable for checking English bigrams frequency? We want to assess the frequency of different English graphemes. Most of the databases that we have checked seem to...

18 February 2021 4,565 3 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Defining effective diffusivity in porous zone and non-porous zone?

Hi all, I am working on methanol steam reforming using packed bed for hydrogen generation. I am using FLUENT software for CFD simulation. In reference paper, authors mentioned that effective mass...

10 February 2021 6,104 1 View