Lance Girton

5 Questions 3 Answers 0 Followers

Questions related from Lance Girton

FLAG tag ScFV with or without a linker?

I am trying to FLAG tag the N terminus of an ScFv construct. Should I add a short linker between the FLAG and the scFv?

07 February 2019 7,357 1 View

ICS for IL-8 in neutrophils?

I am trying to evaluate neutrophil cytokine production in responses to bacterial PAMPs. I have conducted ICS on monocytes and get great results. Unfortunately, I cannot get a positive stain for...

16 November 2017 7,623 2 View

Does anyone have a protocol for lysing purified lysosomes using sonication? I want to avoid detergent because I will be using the lysosomal contents.

I can purify the lysosomes just fine. I just need a recommendation of parameters for sonication. Thanks in advance!

08 August 2017 7,141 1 View

How can I raise the pH of a potassium phosphate buffer from ~6.2 to ~7.5?

I know there must be a formula. I'd prefer not to use NaOH if possible.

02 May 2016 9,659 6 View

Does treating monocytes for 12 hours with EGTA influence their ability to produce cytokines through Fc gamma receptor phagocytosis?

I know that extracellular calcium can replenish intracellular calcium stores. However, they myriad of papers on the issue appear to be contradictory as to the requirement for extracellular calcium...

18 September 2015 8,705 3 View