Who has analyzed essential oils in hemp and/or hops on HPLC?

I am trying to utilize my equipment as efficient as possible and want to try to run terpenenes (essential oils) on hemp and hops. Has anyone tried this before or does anyone know whom to contact...

17 August 2020 7,267 6 View

Where can I find academic books on sustainability and also graded/extended reading?

I am purchasing books from my designated budget. Academic books on either sustainability, UNESCO 2030 goals, and also graded and extended reading. Any suggestions would be very helpful and...

09 March 2020 4,723 5 View

Can a cross-over design control for regression toward the mean?

04 March 2020 3,302 3 View

Importance of light chain type on isotype control antibodies for flow cytometry?

I am putting together a panel for flow cytometry, for one particular antibody the only available isotype control has a different light chain to the test antibody. My test antibody is BV711-CD25...

10 June 2019 3,466 2 View

Can anyone explain how I choose a surfactant for a water in oil macroemulsion, and how I decide what volumes of each substance to use?

I am trying to create a water in oil macroemulsion with droplet sizes as large as possible (hoping to get between 0.1-1mm). I have no idea what surfactant to use or the volume of each phase to...

31 May 2019 7,376 3 View

I am planning to do a project management study that is related to health. What is the best topic to research on?

Any suggestions i will appreciate

06 April 2019 7,158 8 View

Optimising RT-qPCR for the detection of very low abundace IL-17A transcripts from human PBMC mRNA?

I am performing qPCR to quantify cytokine expression in human peripheral blood mononuclear cells. I have found it very difficult to get consistent results when assaying for IL-17A. Currently I am...

23 January 2019 8,404 1 View

Can DNase treatments to RNA preparations cause damage or loss to the mRNA?

When performing qPCR, I have noticed that cDNA made from RNA preparations treated with Turbo DNase (Invitrogen) prior to reverse transcription produce CT values that are shifted several cycles...

31 October 2018 9,688 5 View

Deaf friends in Dar es Salaam have asked: What research has been done on the signed language(s) used in Moshi and Arusha, Tanzania?

My Deaf friends in Dar es Salaam, Tanzania have told me the the sign language used in Moshi and Arusha is different than the signed languages used in other parts of Tanzania. They asked me to...

11 July 2018 3,549 1 View

Is a cell self-conscious?

10 September 2017 5,686 88 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

No title

02 March 2021 3,060 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View