Which companies sells ELISA kits (relating to cost/benefit) to measure murine lipoxin A4 in plasma and tissue and the best way to collect lipoxin A4?

Hello, I am looking for companies that sell ELISA kits to measure murine lipoxin A4. I just found a few, but they are quite expensive (about 600 dollars ish). Does anyone know a company that sells...

09 October 2014 2,660 3 View

Doubts about staining for CD204 IN C57BL/6 mice - can anyone help?

Hello everyone, I have doubts for staining for CD204 (SR-A or MSR1) in C57BL/6 mice, does anyone know a company that sells this antibody for flow cytometry that works in C57BL/6 mice? It seems...

06 July 2014 4,723 4 View

How do you disrupt interaction among dendritic cells from T cells?

I am having problems in my sortings of CD8+ DC from spleens of chronically infected mice. I did a gene expression array and, after analysis, I identified a lot of T cell markers (CD3e, Thy1, CD8b,...

02 March 2014 7,299 6 View

Does anyone know a good protocol to generate OVA peptide-pulsed dendritic cells (DC) in vitro?

Hello, I would like to know if anyone has a good protocol how to generate pulsed (DC) in vitro. I have some doubts about to maximize the procedure: 1) Are bone marrow derived DC better to be...

01 February 2014 1,016 11 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View