Event study linear regression model using stata?

Dear all, I read previous posts about event study, but I still cannot find my starting point for coding my event study model in Stata. I really appreciate it if someone could help me with...

22 January 2021 6,885 8 View

Why am I getting multiple bands in western blot (in Isolating adhesion complexes) ?

Hello everyone, I am isolating adhesion complexes and wanted to confirm the presence of a specific protein in the isolated complex. When I performed western bott, I got a band at the right size...

19 September 2020 538 7 View

Does anyone have a MATLAB code of PV module? (Plotting the module's output)?

Hello, I am trying to model a PV module in MATLAB that can get 24h data inputs of (irradiance, air temperature and wind speed) to produce the output.

02 July 2020 5,890 2 View

What's the best protocol for diluting DMSO when thawing the primary human epithelial cells?

Hi, I have some problem with the viability of my primary epithelial cells after thawing. I have used two protocols (centrifuging or only diluting 10 times more and changing the media the day...

03 October 2019 7,990 6 View

How can i build a bypassing circuit for my buck converter when the voltage from the battery is lower than the desired output voltage?

my dc-dc converter input from the battery has a wide voltage range (8-30vdc)

03 April 2019 3,047 3 View

Numerical Analysis of falling film on inclined plate?

looking for journals or research related to Numerical Analysis of falling film on inclined plate.

09 April 2018 459 2 View

Has anyone ever used puppets to assess self-image/self-perception of children?

I aim to launch a comparison study between utilizing puppetry and drawing to assess self-image of children in hospital

08 February 2018 8,938 2 View

Which tissue has highest expression of sdf-1, cxcr7 and cxcr4 in rat normal tissue?

10 January 2016 7,147 2 View

Any advice with non-parametric regression?

I am analysing variables that are not normally distributed and I use PROCESS to predict DV through the mediator variable. Are there any options with non-parametric tests or is it advisable that I...

18 November 2013 2,638 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View