Does anyone have a protocol for Arginase 1 activity assay?

18 September 2013 1 4K Report

I will be using macrophages.

Hello Boram!

I'm actually about to test for arginase 1 activity in bone marrow-derived macrophages myself. Here's a detailed protocol that I will be using:

http://download.springer.com/static/pdf/635/chp%253A10.1007%252F978-1-59745-396-7_3.pdf?auth66=1408038253_009d0188af10e6a880b78038566822cf&ext=.pdf

(see Section 3.3)

The protocol is from the following paper:

J Immunol. 1999 Oct 1;163(7):3771-7.

Th1/Th2-regulated expression of arginase isoforms in murine macrophages and dendritic cells.

Munder M1, Eichmann K, Morán JM, Centeno F, Soler G, Modolell M.

Good luck!

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