Boram Ham

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Questions related from Boram Ham

Any advice on Lysis buffer for MDSC (previous to arginase assay)?

I am trying to do an arginase assay on MDSCs. The protocol I have asks for pepstatin A and leupeptin for the lysis buffer. However, I do not have these protease inhibitors individually but I have...

16 December 2013 4,518 1 View

Does anyone have a protocol for Arginase 1 activity assay?

I will be using macrophages.

18 September 2013 3,814 1 View

Could I substitute Optiprep for Percoll during cell isolation?

I am doing a intrahepatic immune cell isolation. They ask for 40% optiprep but I currently only have percoll. Could I just use percoll instead ? What is the benefit of using optiprep?

22 May 2013 956 3 View