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Hello, I am working on modelling a truncated protein (E520* for example) due to a stop-gain mutation. I am trying to show how the protein structure changes and I need to calculate back-bone...
23 July 2023 1,713 0 View
A549 cells were attempted to be drug-resistant by exposure to drug treatments for a total of 5 months. After the applications, the cells were tried to be removed by trypsinization but could not be...
19 July 2022 6,145 3 View
Hi everyone! I would like to run mediation analysis but my independent variable motivation (motivated: 1, control: 2) which has two levels. The predictor and dependent variables are continuous. Is...
24 April 2022 8,644 2 View
Hi everyone, Is there any way to conduct bootstrapping for repeated measueres ANOVA in SPPS? (I am using versionn 25) I cannot see any bootstrapping option under for general linear model-repeated...
17 April 2022 7,350 3 View
Hi, I was planning to conduct mixed ANOVA for my analysis for my data since I have two within factors and one between subject factor variable. However, my data is not normally distributed and...
15 April 2022 6,994 3 View
Dear all, I have an epidemiological questionnaire for a project that I am currently involved. We need to use a web-based questionnaire for the patients, but the questionnaire will include...
07 March 2022 8,316 4 View
Dear all, I am looking for a database, or a web-based tool which takes the name of a disease (Alzheimer's disease, as an example)as the input, and retrieves all pathways related to Alzheimer's...
30 January 2022 8,245 3 View
We have a glass reactor to have a silica surface modification. We introduce a solution of silica in TMB and start stirring. But after a while silica is attached to the wall. Do you have an idea...
09 May 2021 4,004 2 View
Hello, Does anyone have a detailed protocol for H&E histological scoring of acute inflammation in particularly "trachea"? Thank you. Nazli
05 July 2020 8,274 3 View
We do this for SARS-COV-2 detection from patients' swap samples. The virus is showing fluctuations in copy number in progress and there is no standard sampling, so we have trouble in deciding...
13 April 2020 4,106 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
