Can I recover RNA/DNA/protein from dead cells?

11 December 2016 8,644 3 View

What yield should I expect for RNA extraction from human PBMCs?

I'm extracting RNA from PBMCs, and do not know what constitutes a normal yield. What is normal for your lab, what method do you use (TRIzol, RNeasy, etc.), and how many input cells do you use?...

11 December 2016 2,963 9 View

Why are my cells dying in MLR?

09 October 2016 648 4 View

Why are these protein isoforms conserved in vertebrates?

I study two protein isoforms of WNT5A that differ from one another by only 18 amino acids post-processing. These isoforms are highly conserved among vertebrates. We see no evidence that these...

02 March 2016 6,576 3 View

Under what conditions should I exclude a Western blot (of total and phosphoproteins) in the analysis of signaling pathway activation?

I've been blotting samples derived from cells that were treated with a ligand. I expect this ligand to activate a signaling pathway, and I expect certain components of this pathway to be...

01 February 2016 4,294 5 View

Why am I getting so many non-specific bands on my western blot?

31 December 2015 8,197 7 View

How can I determine tissue/disease state specific expression of protein isoforms?

A question from my PI:"We would like to search a RNA-seq database(s) using a sequence to determine what tissues, cells etc a particular transcript is expressed in. I looked on the NCBI and UCSC...

11 December 2015 5,519 3 View

What could cause a negative band in WB?

I'm blotting against STAT3 and pSTAT3Y710. My pSTAT3 antibody works great, but I observed a negative band (a white band that is lower intensity than background) at the predicted MW for STAT3 when...

10 November 2015 2,545 7 View

How do I calculate statistical significance for ChIP fold enrichment?

I have some mean amount of DNA from my ChIP assay (A), and some mean amount from the IgG (B). Call fold enrichment C. C=A/B I have standard deviations for both A and B. However, If I calculate the...

05 June 2015 1,548 6 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View