Frances Phalan

3 Questions 3 Answers 0 Followers

Questions related from Frances Phalan

DOes anyone have a good tissue dissociation protocol for FACs?

I would like to dissociate epithelial cells from zebrafish tails and then do FACs and eventually RNA Seq. Does anyone have a good protocol that will give me good dissociation without destroying...

03 March 2016 9,106 2 View

Can anyone recommend a good DNA ladder that can be used for products from 100bp to 20kb?

I am looking for an easy to read ladder especially on the upper end. I have a ladder that currently goes up to 12kb but after 5kb the bands are hard to decipher.

01 January 2016 3,490 4 View

Anyone have luck with the Surveyor Mutation Detection Kit from IDT?

I am trying to do SNP genotyping using this kit but it seems our PCR buffer isn't compatible with this kit...Does anyone have any advice?

01 January 2016 7,587 2 View