How anxiety can be separated from curiosity in open field test for mice?

It is seen that in open field test mice do rearing more suggesting they are more exploratory or curious. Now it is considered one of the markers of anxiety like behaviour. But it is curiosity I...

01 February 2019 1,157 2 View

Elevated plus maze VS. open field anxiety test?

For the detection of anxiety of mouse which test is more suitable and why? Thanks, MS

01 February 2019 2,780 1 View

Meaning of increased rearing and grooming?

I have found my mice showed more rearing and grooming but my question is why they do that more and what does it indicates? Thanks MS

01 February 2019 4,927 7 View

Hyperactivity in mouse?

How the hyperactivity of mouse can be measured and how we can understand that these mice are hyperactive? Thanks MS

01 February 2019 1,573 1 View

When is the best time to do mice behavior test?

Mice are naturally nocturnal but most of the lab behavioral tests are conducted in the day time. Is this confounding? Therefore when is the best time to do behavioral test of mice/rodents? Thanks...

31 December 2018 509 4 View

How many behavioral tests can be conducted in parallel in mice?

For some reasons most of the time multiple behavioral tests such as open field, rotarod, walking beam, and novel object recognition need to conduct in parallel. However, there may be time issue...

31 December 2018 8,055 3 View

Why oligodendrocytes behave differently in different parts of the CNS?

It is observed that oligodendrocytes functions differs based on its position in CNS, why?

31 December 2018 3,358 2 View

Which is better test to assess anxiety in mouse?

Dears, To test anxiety and locomotion commonly "Open filed" and "Elevated-plus maze" apparatus are used. However, for certain reasons multiple instruments is not possible to use in same study. To...

31 December 2018 4,901 4 View

Activator of B or T cells

31 December 2016 2,492 0 View

Antibody for CD8 T cells for immunohistochemistry/flurescence....

07 August 2016 6,347 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

How to show arrows over the streamlines in CFD-post?

I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...

01 March 2021 8,668 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View