Does anyone have a good MTT assay protocol for non-adherent cells?

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Rafik Karaman

Dear Yogi,

You may use the protocol contained in the following link:

MTT Cell Proliferation Assay ATCC® 30-1010K

https://www.atcc.org/~/media/DA5285A1F52C414E864C966FD78C9A79.ashx

Hoping this will be helpful,

Rafik

Saleh Alkarim

Hello

Follow the protocol in this attachment

Muruganandan Shanmugam

You can centrifuge the cells after completing the incubation period with MTT. Remove the supernatant and read the pellet after dissolving.