Does anyone have a good method to analyze the necrotic core area by using heart frozen section?

11 November 2022 1 6K Report

Hello everyone,

I have heart frozen sections that I need to use for measuring necrotic core area,

Mostly I stained for Oil-red O but I found it very confusing when it comes to calculate the necrotic core area. Does anyone have any suggesting or software that can be used?

Many many thanks.

Joseph Westaby

I would fix them in 10% formalin and stain with H&E. It should be easy to appreciate necrotic areas. You could then digitally scan the slides and use image application software such as ImageJ or Visiopharm to quantify. I've not used Oil-red O to quantify necrosis before. I have heard of 2, 3, 5-triphenyte-trazoliumchloride being used but have no experience with this myself.

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