I have been trying elecroporation with siRNA in pancreatic islets unsuccessfully. So I am trying in primary beta cells, but after that I need to perform GSIS in this cells.
You may find the following thread useful:
https://www.researchgate.net/post/Does_anyone_have_a_full_detailed_protocol_for_GSIS_in_primary_isolated_mouse_islets#view=559edcd66307d990258b456d
Nayara, I am not sure you can get good results with siRNA + electroporation in pancreatic islets. We have used electroporation only for cell lines.
You could transfect your islets with lentivirus containing shRNA instead. Take a look into this paper: http://www.ncbi.nlm.nih.gov/pubmed/21078968
Are you sorting your primary beta-cells with FACS?
Hi Nayara
I wonder if you found a useful way for successfully transecting your islets? as I am having the same issue.
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