Error While Solving Stiff Problems In COMSOL?

Hi, So I am trying to solve a stiff problem using Global ODEs and DAEs. The problem has 32 differential equations and I have inserted all the initial values correctly, however, I keep getting this...

28 January 2021 1,872 3 View

How to solubilize protein?

Hello, I have cloned my gene of interest in a bacterial expression system i.e. BL 21 Gold (DE3) and checked for protein production and solubility. Good amount of protein was observed on SDS-PAGE...

15 January 2021 7,063 5 View

What is the influence of reduced graphene oxide (rGO) in a p-n ternary photocatalyst?

04 December 2020 9,019 2 View

Why is the protein of interest soluble, but inactive?

Hello, I have cloned and expressed bacterial protein in E. coli system in the soluble fraction. However, on performing the activity assay, no activity with the substrate is seen. The standard...

24 November 2020 8,012 5 View

Is It Possible to Produce Yellowish Graphitic Carbon Nitride Using Urea as a Precusor?

Hello, Recently I have been attempting to synthesize graphitic carbon nitride for the purpose of photocatalytic conversion of carbon dioxide to methane under visible light. The issue I am facing...

12 November 2020 6,854 4 View

How Important Is the Rate of Heating During the Synthesis of Carbon Nitride?

09 November 2020 6,096 2 View

What is the influence of rate of heating on synthesis of graphitic carbon nitride (g-CN)?

16 October 2020 4,216 4 View

Can you expect to see changes in secondary structure in a MD simulation?

While running a simulation on any peptide or protein, is it possible for the secondary structures to change? For example, seeing a steady rise in helical content over the whole simulation?

30 September 2020 4,298 9 View

What is the sensitivity of SHM technique based on electromechanical impedance method to identify a crack in a concrete beam?

The sensitivity of the technique is important to know how to identify the appearance of cracks

17 August 2020 4,156 9 View

How to export data from static analysis in Ansys Workbench to excel?

Hello, I am running a static analysis for 10 time steps (variable load) in Ansys workbench 19.2. I would like to export to excel the nodal stress everywhere in the structure (not just the maximum...

17 August 2020 6,105 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View