Does anyone has working antibodies for immediate early gene expression (c-fos and/or ZENK) in bird brains that are NOT the ones from Santa Cruz?

More Cristián Gutiérrez-Ibáñez's questions See All

Which preprint platforms or servers are your preferred choice for Health Sciences?

I would like to know other researchers opinion about their preferred preprint servers/platforms

28 May 2024 8,291 0 View

How can we design 2d HEXAGONAL Superlattices having self-similar clusters composed of heptamers ?

Imagine a 2d Hexagonal Superlattice composed of regular heptamers, where every site in the heptamer is not a particle but an embedded heptamer and so on.. what isotropic potential self-assemble a...

23 September 2023 542 0 View

Is it possible to design 3D nanoparticle Superlattices of clusters in the self-assembling of colloidal particles via purely repulsive interactions?

Colloidal particles interacting via purely repulsive interactions self-assemble in 2d hexagonal Superlattices composed of clusters such as dimers, trimers tetramers and more....what happens in...

13 June 2023 7,144 1 View

Do you know protocols to work with macrophage primary cell culture from mouse?

Any suggestion, recommendations, things I must know. Thankyou very much.

06 March 2023 7,032 1 View

How to correct PAM fluorometry measurements when the algal tissue is smaller than the measured area by the spectrometer?

In a study in which we want to study the rhodophyte Gracilaria vermiculophylla with PAM fluorometry (diving PAM) we are facing the problem that this small tubular algae doesn't cover the complete...

08 February 2023 2,202 2 View

What is the relation between thrust coefficient curves and turbulence intensity in wind turbines?

I have witnessed that higher values of TI lead to a decay in the Ct curves in the moderate range of wind speed (8-13 m/s). For high wind speeds it is seen that the changing TI does not have an...

06 December 2022 9,289 1 View

What is the relation between thrust coefficient curves and air density in wind turbines?

I came with the following explanation, but I am not sure if it is correct. The variation of the thrust coefficient is directly related to the variation of the thrust force. According to the...

04 December 2022 4,279 1 View

Does anybody has the biodot zx1010 manual?

Hello! i´m trying to use the biodot zx1010 to make the test and control line on a lateral flow strip but the manual that came to our machine it isn´t the right one, and doesn´t have much...

25 January 2021 6,790 1 View

If the daily (hourly) concentration of PM2.5 is not Gaussian. Is correct apply an arithmetic mean in epidemiological studies for risk assessment?

The distribution of daily PM2.5 concentration (hourly) is bimodal in the city of Coyhaique (South of Chile). Coyhaique is the city more polluted by particulate matter accord the WHO. The major...

11 October 2020 2,310 3 View

How is the zeta potential measured in sol-gel solutions?

I try to measure the zeta potential of sol-gel solutions but I never get good results, each time I get a different value. My formulations are between 75-95% alcohol and I use a zetasizer.

19 September 2019 3,710 4 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

Noor Edan Jarad

hi see this article

Article Food for Song: Expression of C-Fos and ZENK in the Zebra Fin...

Cristián Gutiérrez-Ibáñez

Thank you Noor Edan Jarad , but those are the same as I mentioned before, they used a different code for them. K-25=sc-253, C-19 = sc-189

Kevin Broad

I tried the abcam polyclonal rabbitt one in a chicken brain once I think it was 209794 worked OK but don't overfix