Does anybody has the biodot zx1010 manual?

25 January 2021 6,257 1 View

If the daily (hourly) concentration of PM2.5 is not Gaussian. Is correct apply an arithmetic mean in epidemiological studies for risk assessment?

The distribution of daily PM2.5 concentration (hourly) is bimodal in the city of Coyhaique (South of Chile). Coyhaique is the city more polluted by particulate matter accord the WHO. The major...

11 October 2020 2,006 3 View

Does anyone has working antibodies for immediate early gene expression (c-fos and/or ZENK) in bird brains that are NOT the ones from Santa Cruz?

Hi Given that Santa Cruz has discontinued the antibodies for c-fos ( sc-253 ) and egr-1 (SC-189), which where widely used in birds, does anyone has working alternatives? Thank you.

17 December 2017 9,316 4 View

Is there a survey about the economic situation and activity of professional visual artists in your country?

I am currently working on the economic activity of Spanish professional artists, and have already published the first conclussions of an extensive survey, including data from over 1100 artists in...

11 December 2017 3,503 3 View

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19 November 2015 510 9 View

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10 November 2015 1,293 10 View

Is there any (sub)command in SPSS to perform MIXED model for multivariate (4DV) repeated measures?

Currently, I am looking for this in order to improve my findings. It is possible? Thank you!!!

10 September 2015 4,237 3 View

How can I estimate the error on x [y=max(x)] in a quadratic regression?

Hi all, I would like to estimated the elevation at which species richness is expected to be maximal. To this, I used a quadratic relationship, y = a*x^2 + b*x + c Where y is the species richness...

19 August 2015 9,476 3 View

Protocol for extracting miRNA - can anyone help?

I am looking for a protocol for extracting miRNA and RNA, avoiding the use of a commercial kit.

30 September 2013 3,518 4 View

What is the best reference miRNA in qPCR of miRNA when working with human cancer cell lines?

I am having some trouble with RNU6B as endogenous control for several ovarian cancer cell lines. Could you recommend me other stable reference miRNAs that have worked in your experimental approaches?

20 June 2013 890 19 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View