What is the appropriate protocol for generation of Dendritic cells from mouse bone marrow..should it be GM-CSF+IL-4 or only GM-CSF?

Hi everyone, I am trying to generate Dendritic cells from mouse bone marrow where I am culturing the bone marrow cells with GM-CSF for 6 days and measuring the percent DCs generated on 6th day by...

02 March 2019 1,911 8 View

Will the standard protein available in ELISA kit be treated as reagent in other assays?

Hello all, I have IFN-alpha kit, which is having IFN-ALPHA protein as its standard. Can this protein be treated as reagent and used as inhibitory or stimulatory agent in other assays like...

11 December 2016 3,680 6 View

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Hello all, I have supernatants containing IFN-alpha protein at concentrations in pg/ml...I want to use them in PBMC stimulation assays...kindly tell me conversion procedure of pg/ml to IU for...

09 October 2016 6,213 2 View

What should be time of detection for phosphorylation of IKK-alpha after TLR-7 activation in pDCs?

Hi all, I am working on pDCs in HIV-1 infection. I would like to detect phosphoryaltion of IKK-alpha after TLR-7 activation of pure pDCS by TLR-7 agonist. Afetr how much time should I fix the...

08 September 2016 9,688 2 View

How should I stimulate plasmacytoid dendritic cells to induce phosphorylation of akt?

Hello, I am working on freshly isolated plasmacytoid dendritic cells. I stimulate them with Imiquimod (TLR-7 agonist) to see the phosphorylation of akt (ser473) after 90 min by flow...

02 March 2015 9,145 4 View

Which biosafety lavel should be used for using flu PR8 virus (H1N1) as stimulation of plasmacytoid dendritic cells?

I am working on plasmacytoid dendritic cells and many references suggest that flu PR8 is best for stimulation of pDC...can anyone tell me if I plan to use this virus what kind of biosafety should...

01 February 2015 2,072 9 View

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10 November 2014 200 12 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View