Does anyone has MS-5 mouse stromal cell line?

12 December 2014 0 9K Report

This cell line is available at DSMZ, Germany, but they do not supply cell lines to India. Can anyone help me to obtain this cell line?

Badges
Science topic

More Ashwini Dhamanage's questions See All

What is the appropriate protocol for generation of Dendritic cells from mouse bone marrow..should it be GM-CSF+IL-4 or only GM-CSF?

Hi everyone, I am trying to generate Dendritic cells from mouse bone marrow where I am culturing the bone marrow cells with GM-CSF for 6 days and measuring the percent DCs generated on 6th day by...

02 March 2019 1,992 8 View

Will the standard protein available in ELISA kit be treated as reagent in other assays?

Hello all, I have IFN-alpha kit, which is having IFN-ALPHA protein as its standard. Can this protein be treated as reagent and used as inhibitory or stimulatory agent in other assays like...

11 December 2016 3,737 6 View

Please can anyone tell me how to convert pg/ml IFN-alpha quantity to International Units(IU)?

Hello all, I have supernatants containing IFN-alpha protein at concentrations in pg/ml...I want to use them in PBMC stimulation assays...kindly tell me conversion procedure of pg/ml to IU for...

09 October 2016 6,281 2 View

What should be time of detection for phosphorylation of IKK-alpha after TLR-7 activation in pDCs?

Hi all, I am working on pDCs in HIV-1 infection. I would like to detect phosphoryaltion of IKK-alpha after TLR-7 activation of pure pDCS by TLR-7 agonist. Afetr how much time should I fix the...

08 September 2016 9,758 2 View

How should I stimulate plasmacytoid dendritic cells to induce phosphorylation of akt?

Hello, I am working on freshly isolated plasmacytoid dendritic cells. I stimulate them with Imiquimod (TLR-7 agonist) to see the phosphorylation of akt (ser473) after 90 min by flow...

02 March 2015 9,215 4 View

Which biosafety lavel should be used for using flu PR8 virus (H1N1) as stimulation of plasmacytoid dendritic cells?

I am working on plasmacytoid dendritic cells and many references suggest that flu PR8 is best for stimulation of pDC...can anyone tell me if I plan to use this virus what kind of biosafety should...

01 February 2015 2,249 9 View

Can I use -20°C incubation temperature, when permeabilization of PBMCs is recommended to done on ice?

I am working on effect of HIV-1 on phosphorylation of cytoplasmic proteins to be analyzed by flow cytometry. For staining purpose it is recommended to permeabilize the PBMCs with Perm III buffer...

10 November 2014 268 12 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View