Ashwini Dhamanage

8 Questions 15 Answers 0 Followers

Questions related from Ashwini Dhamanage

What is the appropriate protocol for generation of Dendritic cells from mouse bone marrow..should it be GM-CSF+IL-4 or only GM-CSF?

Hi everyone, I am trying to generate Dendritic cells from mouse bone marrow where I am culturing the bone marrow cells with GM-CSF for 6 days and measuring the percent DCs generated on 6th day by...

03 March 2019 1,972 8 View

Will the standard protein available in ELISA kit be treated as reagent in other assays?

Hello all, I have IFN-alpha kit, which is having IFN-ALPHA protein as its standard. Can this protein be treated as reagent and used as inhibitory or stimulatory agent in other assays like...

12 December 2016 3,715 6 View

Please can anyone tell me how to convert pg/ml IFN-alpha quantity to International Units(IU)?

Hello all, I have supernatants containing IFN-alpha protein at concentrations in pg/ml...I want to use them in PBMC stimulation assays...kindly tell me conversion procedure of pg/ml to IU for...

10 October 2016 6,260 2 View

What should be time of detection for phosphorylation of IKK-alpha after TLR-7 activation in pDCs?

Hi all, I am working on pDCs in HIV-1 infection. I would like to detect phosphoryaltion of IKK-alpha after TLR-7 activation of pure pDCS by TLR-7 agonist. Afetr how much time should I fix the...

09 September 2016 9,727 2 View

How should I stimulate plasmacytoid dendritic cells to induce phosphorylation of akt?

Hello, I am working on freshly isolated plasmacytoid dendritic cells. I stimulate them with Imiquimod (TLR-7 agonist) to see the phosphorylation of akt (ser473) after 90 min by flow...

03 March 2015 9,185 4 View

Which biosafety lavel should be used for using flu PR8 virus (H1N1) as stimulation of plasmacytoid dendritic cells?

I am working on plasmacytoid dendritic cells and many references suggest that flu PR8 is best for stimulation of pDC...can anyone tell me if I plan to use this virus what kind of biosafety should...

02 February 2015 2,187 9 View

Does anyone has MS-5 mouse stromal cell line?

This cell line is available at DSMZ, Germany, but they do not supply cell lines to India. Can anyone help me to obtain this cell line?

12 December 2014 9,402 0 View

Can I use -20°C incubation temperature, when permeabilization of PBMCs is recommended to done on ice?

I am working on effect of HIV-1 on phosphorylation of cytoplasmic proteins to be analyzed by flow cytometry. For staining purpose it is recommended to permeabilize the PBMCs with Perm III buffer...

11 November 2014 240 12 View