I am performing gelatin zymography for MMP-2 & MMP-9 and casein zymography for MMP-3, using protein ladder. If anyone has the standards, can you please spare few microlitres for one gel?
You are free to suggest any other alternative.
Currently I am comparing the band intensity of my disease group with control group, in that case "Is it absolutely necessary to run MMP standards to quantify in a zymogram?".