I am working on a project dealing with glycoproteome profile of patient samples. I enrich the samples using Concanavalin A agarose column followed by sample preparation for LC-MS (Orbitrap). The data analysis gives me the list of proteins and their abundance in the corresponding sample.
How can i get the site of glycosylation and type of moiety attached?
I know that Byonic software is used to analyze glycoproteome, however, its too costly to afford. Kindly suggest alternatives.
Thanks in advance.