Does anyone has experience or idea in isolating single B cells from rabbit?

Thamaraikannan Paramasivam @Thamaraikannan-Paramasivam

27 March 2017 1 1K Report

How to isolate single B cells from Rabbits and use it for antibody development

Hi Jing Zang

I dont want to go for the hybridoma method, as this method of antibody has a lot to lot variation issue. I want to generate a recombinant monoclonal by isolating single B cell from immunized rabbit and then use the mRNA from this for generating recombinant clones

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Thamaraikannan Paramasivam's questions See All

While performing MD simulation, is it possible to add other ions while solvating in addition to 0.15 M of NaCl and MgCl2?

Can an MD simulation be performed by adding other salts by varying their concentration inside the box?

01 May 2023 9,230 3 View

How can be the protein immobilized s-CM5 sensor chip stored?

I have stored the cm5 chip in 50 mL of HBS-EP buffer pH 7.0 at 4°C... which buffer can be used for long-term storage of the chip?

10 April 2023 4,319 2 View

In a MD simulation, a protein complex with > 600 amino acid can have a RMSD over 1 nm (10 Angstorm)?

MD simulation of a protein complex with more than 600 AA residues shows an average rmsd between 1-1.5 nm. can the protein complex can be considered stable? Besides sometimes i see a jump in the...

06 November 2022 6,302 4 View

Is it possible to indulge a dimeric protein (Target) into docking against another protein (Bound to a metal ion) using HADDOCK server?

I have been using CLUSPro 2.0 for protein-protein docking, but it does not take the metal ion into consideration because it selectively takes the protein chain. In contrast, HADDOCK considers the...

06 June 2022 3,285 4 View

Is there any way/tools to interepret the molecular dynamics simulation results beside XmGrace?

I have done MD simulation and want to interpret and plot the data from the files. As my PC did not have enough specs for Dual boot OS because the XmGrace tool is supported only on Linux. Though, I...

25 April 2022 4,254 3 View

Whether ethical committe (IAEC) approval is needed for samples procured from butcher shop?

I am devising a study to evaluate the invitro anti- resorptive effect of my sample in bovine bone procured from butcher shops/markets. Though there are studies in which the samples from...

20 September 2021 4,259 6 View

How to find the amino acids which are located in the particular protein binding site?

As i am working on protein-ligand docking, i want to input the x,y,z coordinates in autodock and to do that, i need the coordinates of aminoacids present in the binding site. So, PLease help me out.

03 January 2021 4,099 4 View

Importance of distance education?

Distance learning is a learning method in which students are not required to be physically present in a classroom setting or school.

25 December 2020 8,704 10 View

Farming is an non profitable occupation ? If agree / disagee reasons?

Some of the problems faced by farmers..... 1. Poor marketing channel 2. Farmers not getting remunerate price for the own produce 3. Lack of bargaining power (someone fix the price) 4. Monsoon...

30 November 2020 3,819 4 View

Difference on natural farming and organic farming???

Based on my perception, organic farming we can use organic based fertilizers like biofertilizers, Azolla, Panchakavya, Neem oil extract etc. Whereas, in natural farming no application of any kind...

23 November 2020 7,080 22 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Is perfusion required to stain brain tissue with every method?

I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...

14 July 2024 177 4 View

Does anybody have Anti-Claudin8 and Anti-Claudin2 Primary conjugated Ab (Target Species: Human)?

I need Anti-Claudin8 Primary and Anti-Claudin2 conjugated Ab for my immunofluorescence experiment. If anybody has worked on it. Please share the accession number/details.

14 July 2024 485 0 View

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized?

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...

13 July 2024 2,431 2 View

Can I use BSA for a Histological Blocking Solution?

Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...

07 July 2024 7,967 3 View