The dried senna alata leaf is brittle and hard to remove the epidermal peel. I am aim to observe the stomata guard cells under microscope.
For the preparation of epidermal peel of dried senna alata leaf, use of 0.5% HCL is effective for 12hrs.
Hi Sayyed lliyas, does that meant I need to immerse the dried sample into 0.5% HCL solution for 12 hours in order to soften the tissue ?
I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....
01 March 2021 3,325 2 View
I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...
17 February 2021 3,506 4 View
All, I am trying to equilibrate an Ice 1h crystalline structure to 250K in LAMMPS. When I tried to do this at 250K directly, the structure lost it's crystalline shape and became disordered post...
14 February 2021 5,747 2 View
Trying to simulate a ice-PTFE interaction and am unable to find a reference which actually lists out forcefield parameters for PTFE-PTFE interactions using ReaxFF or even other force fields like...
08 February 2021 9,373 2 View
1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.
04 February 2021 9,069 3 View
I have created an Ice 1h structure and am equilibrating it 100K in NVT ensemble in LAMMPS for a timestep of 0.25fs in 3000 steps. The potential used is TIP4P/Ice. Although the simulation runs fine...
26 January 2021 9,603 11 View
Hello, I'm trying to select fluorophores for a large panel of flow cytometry, so I'm wondering what is the maximum degree of overlap between two fluorophores that is considered acceptable? Thanks.
19 January 2021 3,475 3 View
Hi everyone, I would like to run a Kruskal-Wallis test on a large sample with a likert scale. I am having difficulties figuring out how to process with the ''rank_avg'', and rank sum with such a...
05 December 2020 793 3 View
Hello everyone, When I put a wet sample in a vacuum desiccator, the larger pores tend to empty. I would like to know if there is a relationship between vacuum pressure and the size of emptied...
25 November 2020 9,967 5 View
I have 2 sets of regression estimations with same dependent variables, same control variables and model. In the second regression, there are two additional variables. However, the sign and...
28 September 2020 6,304 6 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View