Have you used Cyrene (dihydrolevoglucosenone) to dissolve PVDF?

I noticed in C&EN that a bio-derived solvent called Cyrene has been developed in hopes of it replacing DMF or NMP. Has anyone tried to dissolve PVDF in Cyrene? If so, what were your results?...

23 February 2020 8,032 2 View

R: How to cut off / drop rows of a column after reaching a specific value the first time?

I have the following problem: In a table with multiple coulumns I want to cut off / drop / disable all rows of one column before and after reaching specific values. The tricky part is, that "="...

16 February 2020 2,288 4 View

What are typical safety values for electrical current and field strengths in active implantable devices?

08 August 2017 9,441 2 View

What are typical safety values for electrical current and field strengths in active implantable devices?

07 August 2017 8,887 2 View

Contact dermatitis to ECG electrodes during long-term wear

What is the percentage number of people (in a clincial sub-population) developing irritant or allergic contact dermatitis to wet ECG electrodes? Are there gender differences or differences between...

06 July 2017 3,523 3 View

Does anybody know the light absorption coefficient of sebum and sweat?

20 March 2017 2,530 1 View

Does anybody know the light absorption coefficient of sebum and sweat?

02 March 2017 1,454 1 View

Can someone identify these symptoms in lemon leaves??

Plants are young (tree nursery). Apical leaves are affected.

30 September 2016 9,256 13 View

Is there a method for background subtraction or a way to compensate differences between two electrodes due to production in EIS measurements ?

We would like to develop a EIS based protocol for detection of an antibody. For this test we will need to measure two electrodes. One was incubated with the sample and the other with a...

05 June 2016 7,828 6 View

Is anybody interested in a collaboration to test the first medical avatar of the human arterial tree?

16 April 2016 9,172 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View