SPSS - how to create new variable include and excluding cases based on another variable?

Hi there, I have been searching and searching and cannot find an answer to this problem... I have a variable (Ref_Accepted_Comb) for the number of referrals accepted with values of (1=Yes, 2=No,...

08 February 2021 3,479 3 View

Does maternal potentiation work?

Has anybody been able to replicate finding of papers that show increased vocalization in mice pups after a brief period of interaction with their dam, also called maternal potentiation? We have...

11 January 2021 558 2 View

Anybody know how can I analyze differences between groups vocalization syntax using the transition probability between syllables?

I have been using a MATLAB script called DeepSqueak to analyze vocal communication in mice. One of the outcomes of this analysis is the transition probability between the different syllables that...

09 December 2020 1,525 1 View

Anyone with experience conducting proximity ligation assay (PLA) in mouse heart tissue ?

I was wondering if anyone had any experience doing proximity ligation assays (PLA) using mouse whole heart (ventricular) tissue (in any state)?

29 November 2020 9,103 1 View

How to extract/remove ethanol from kava tincture?

Producing Kava tincture using different alcohol concentrstion as solvent, soaked for 3 weeks filter off the kava paste and left with mixture of kavalactones and ethanol..need a method to remove...

14 June 2020 5,104 1 View

Can you have one variable load onto two factors in a CFA, prior to SEM?

I am running a SEM, and I have 6 variables which will load onto two factors (positive and negative mental health). However, this model does not meet a good model fit. When I allow depression to...

19 May 2020 8,286 3 View

When does someone use corrected fin length and the actual fin length in heat transfer problems?

In some instances the extended length of a fin is used but then in other instances the normal fin length is used in extended surface heat transfer problems.

23 March 2020 7,553 4 View

How will I calculate the surface temperature of a HDPE pipe that is exposed to the sun?

I need to calculate the final temperature of a pipe that's been lying in the sun for the day, however, I am unsure how to do this. I know how to calculate the heat transfer through the pipe wall...

19 January 2020 1,259 10 View

Mouse islet isolation without pancreas perfusion?

Is there a current/reliable way to isolate mouse islets (keeping their morphology and viability for culturing in vitro) without perfusing the pancreas through the bile duct?

14 September 2019 3,577 1 View

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19 October 2018 6,019 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View