Hi, guys. I have a question about the execution of polysome gradient. In my experiment, only disome is showed. What is happening?

22 March 2023 0 6K Report

My experiment showed only one peak for polysome, but I expected to see several peaks referring to the polysomes. What is happening? I used a 7-47% sucrose gradient. Fifteen units of OD254 were applied and centrifuged for 3 hours at 4°C at a speed of 39,000 RPM.

Badges
Science topic

More Bruno Roberto Queiroz's questions See All

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

What is meant by baseline of FTIR data?

I got comment on my FTIR data figure from a reviewer. The reviewer said "FTIR data in Figure should be repeated. there is no bassline." I made Y off set comparison graph of FTIR on OriginLab. Can...

03 August 2024 6,070 3 View

What is Random Audit?

HI there, I've came across several articles discuss about random audit an Non random to tax evasion or compliance. Most of the articles is relating about effect of audit (random or non random)...

31 July 2024 5,309 7 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

Can biogeographical regions work as a character in a character matrix for a phylogenetic analysis?

Basically I'm helping with the matrix using a cosmolitan group, could one of the characters used for be biogeographical regions (Neartic, Neotropical, etc)? Most of the data in this matrix refers...

25 July 2024 9,753 2 View

Dataset of synchronized cardiac angiography and ECG?

Hello, I'm working on medical project and I would need synchronized angiography with ECG? Does anyone know if some open source dataset of this kind exist? Regards, Bruno

25 July 2024 2,214 2 View

How we can use lattice-based cryptography for construction of S boxes?

Please suggest basic literature on lattice-based cryptography. A kind response from the cryptographic community will be highly appreciated.

24 July 2024 6,291 4 View

I am working on III-V based tandem solar cells.Can anyone explain that solar cells work under forward or reverse biased conditions?

I want to know that n-doped side of solar cell is connected with positive or negative electrode.

20 July 2024 1,348 2 View

Can it is possible to find the cleaved sequence when a protein cleaved by a heamaglutanin protease (HA/P) by any bioinformatics tools?

Bioinformatics tools like peptide cutter

15 July 2024 6,453 1 View

What is the physical meaning of the magnetic scalar potential?

In cases where the rotational of the magnetic field H is zero, we can define this field as the gradient of a scalar function defined as the magnetic scalar potential (similar to the electric...

21 July 2024 9,633 4 View

Where is the stream gradient usually greatest and relationship between the slope of the stream channel and the velocity of the stream?

13 July 2024 5,825 0 View

How do gradient and discharge change in a downstream direction and why are glacial valleys shaped differently from river valleys?

13 July 2024 9,228 3 View

What happens to stream's discharge if gradient of a stream increases & relationship between gradient of a stream & rate of water flow?

What happens to the stream's discharge if the gradient of a stream increases and relationship between the gradient of a stream and the rate of water flow?

13 July 2024 5,085 2 View

How does that stream's gradient change downstream as it enter the alluvial fan and difference between an alluvial plain and a delta plain?

12 July 2024 7,337 0 View

How does stream gradient steepness change moving downstream and gradient of a stream change along its longitudinal profile?

12 July 2024 4,113 0 View

What is wrong with Site-Directed Mutagenesis?

I am following the NEB protocol to perform SDM with Q5 and NEB HiFi Assembly. I am making a two base pair change. First picture is my Primers designed with NEBaseChanger. Second Picture is my...

24 June 2024 6,079 4 View

Why there are only 3 (not 4) clusters present on my ddPCR EvaGreen different primer concentration duplex assay results?

Hi, I have recently ran a duplex ddPCR EvaGreen assay using different primer concentration for target (150nM) and reference (50nM) primer. The assay was ran on a XY sample for SRY copy number...

22 June 2024 9,200 3 View

Why Q5 DNA Polymerase failed in PCR short primers?

Hi everyone! I am using a Q5 high fidelity DNA polymerase to amplify a 6.7 kb plasmid. The PCR with Q5 went well with longer primers (~55 bases) at 70-72 degree C. However, I keep failing in the...

17 June 2024 1,734 4 View

Effect of cell size on gradient centrifugation?

Hi y'all, does anyone know if animal cells increase in size as they age or do they simply multiply? I usually do a Percoll gradient centrifugation to isolate endothelial cells but with pups it...

07 June 2024 4,065 1 View