I am interested to study effect of a treatment on amyloid beta plaques deposition in transgenic mouse model of AD. Can I use Thioflavin-S Staining method for amyloid beta plaque quantification?
Thioflavin-S is a validate staining method to quantify beta-amyloid plaques in AD brain. You may use reference of Maji SK et al., 2009, Annop A et al., 2014 for the same
It has been our experience in both clinical and research settings that thioflavin will not always pick up the more diffuse beta amyloid deposits. However it will really "shine" in the mature plaques and vascular amyloid in the transgenic mouse model. You might want to do a little pilot study for yourself with thioflavin and antibodies such as 4G8 and 6F/3D in serial section and compare the staining. In humans the result can be quite different with differing areas of the brain! Please see for your reference this interesting article http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286131/
As always, depends on the question that you are asking. The simpler method might be the best.
And less cumbersome for storage of the slides since you do not need to coverslip with an aqueous mounting media as with ThioS.
P.S. Gaurav, my P.I. is from Pisa and is quite noteworthy in neuropathology. Look up Dr. Bernardino Ghetti. We have a number of papers in the area of transgenic mouse model.
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